This tool will calculate the broad-sense heritability for your trait of interest using a set of C. elegans wild isolates. The broad-sense heritability is the amount of trait variance that comes from genetic differences in the assayed group of strains. Generally, it is the ratio of genetic variance to total (genetic plus environmental) variance.

To obtain the best estimate of heritability, please measure a set of at least five wild strains in three independent assays. These assays should use different nematode growths, synchronizations, bacterial food preparations, and any other experimental condition. You should measure trait variance across as many different experimental conditions (in one block) as you would typically encounter in a large experiment.

Please organize your data in a long format, where each row is an independent observation of one strain in one trait. The columns of the data set should be:

1. AssayNumber - a numeric indicator of independent assays.
2. Strain - one of the CeNDR isotype reference strain names.
3. TraitName - a user-supplied name of a trait with no spaces (e.g. BroodSize).
4. Replicate - independent measures of a trait within one independent assay. You can think of this column as a numerical value for a technical replicate.
5. Value - the measured output of the trait (e.g. 297 for BroodSize).

NA values will not be used in broad-sense heritability calculations.